DNA

Part:BBa_K207001:Experience

Designed by: Jennifer Jocz   Group: iGEM09_Harvard   (2009-10-21)


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Applications of BBa_K207001

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UNIQ856ddefe656d0caa-partinfo-00000000-QINU UNIQ856ddefe656d0caa-partinfo-00000001-QINU

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HUST-China 2015

Figure 1: Circuit

Modeling

Before the circuit was determined, there were two kinds of light control system for choice: the CRY2-CIB1 system and the PhyA-FHL system. To find out the system that fits our circuit better, we simulated both of them with the DDEs model.

Figure 2.1: Simulation of PhyA-FHL system
Figure 2.2: Simulation of CRY2-CIB1 system

The figure 2.1 and 2.2 shows the following facts:

1. The values of (active)PhyA, (active)FHL, (active)CRY2, (active)CIB1 are relatively low and remains at a certain level (approximately 0~7nM).

2. The peak of CRY2-CIB1 system appears earlier than the one of PhyA-FHL system.

3. The value of Rox1 in CRY2-CIB1 system decreases faster than the one in PhyA-FHL system.


We can safely derive the following conclusions from the figures above.

1. The photoactive subjects are of low concentration but they remain at a certain level.

2. Compared to the PhyA-FHL system, the CRY2-CIB1 system is more sensitive to light exposure (The peak of CRY2-CIB1 system appears earlier than the one of PhyA-FHL system) and the PhyA-FHL system has a time-lag for photoactivation.

3. The rate of Rox1 degradation in CRY2-CIB1 system is higher than the one in PhyA-FHL system, which means the darkness induction could shut down quickly so that the downstream systems could be activated.

Hence, we considered CRY2-CIB1 system more advantageous and applied it to our project.

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